50. Experimental Biochemistry

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50. Experimental Biochemistry

 

 

CATEGORY: Medical & Medicine – 500 Courses

COURSE NUMBER: 50

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Syllabus

Section I Basic Techniques of Experimental Biochemistry 1
Introduction 3
Experiment 1 Photometry 15
Experiment 2 Chromatography 25
Experiment 3 Radioisotope Techniques 45
Experiment 4 Electrophoresis 61
Section II Proteins and Enzymology 79
Introduction 81
Experiment 5 Acid-Base Properties of Amino Acids 105
Experiment 6 Sequence Determination of a Dipetide 111
Experiment 7 Study of the Properties of ß-Galactosidase 123
Experiment 8 Purification of Glutamate Oxaloacetate Transaminase from Pig Heart 135
Experiment 9 Kinetic and Regulatory Properties of Aspartate Transcarbamylase 149
Experiment 10 Affinity Purificaiton of Glutathione-S-Transferase 157
Section III Biomolecules and Biological Systems 163
Introduction 165
Experiment 11 Microanalysis of Carbohydrate Mixtures by Isotopic Enzymatic, and Colorimetric
Methods 195
Experiment 12 Glcose-1-Phosphate: Enzymatic Formation from Starch and Chemical
Characterization 205
Experiment 13 Isolation and Characterization of Erythrocyte Membranes 217
Experiment 14 Electron Transport 227
Experiment 15 Strudy of the Phosphoryl Group Transfer Cascade Goferning Glucose Metabolism
Allosteric and Covalent Regulation of Enzyme Activity 243
Experiment 16 Experiments in Clinical Biochemistry and Metabolism 253
Section IV Immunochemistry 261
Introduction 263
Experiment 17 Partial purification of a Polyclonal Antibody, Determination of Titer, and
Quantitation of an Antigen Using the ELISA 279
Experiment 18 Western Blot to Identify an Antigen 291
Section V Nucleic Acids 301
Introduction 303
Experiment 19 Isolation of Bacterial DNA 333
Experiment 20 Transformation of a genetic Character with Bacterial DNA 339
Experiment 21 Constructing and Characterizing a Recombinant DNA Plasmid 345
Experiment 22 In Vitro Transcription from a Plasmid Carrying a T7 RNA Polymerase-Specific
Promoter 359

Experiment 23 In Vitro Translation: mRNA tRNA, and Ribosomes 369
Experiment 24 Amplification of a DNA Fragment Using Polymerase Chain Recation 385
Section VI Information Science 397
Introduction 399
Experiment 25 Obtaining and Analyzing Genetic and Protein Sequence Information via the World
Wide Web, Lasergene, and RasMol 405
Appendix Supplies and Reagents 409
Index 437

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