37. Practical Biochemistry

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37. Practical Biochemistry

 

 

CATEGORY: Medical & Medicine – 500 Courses

COURSE NUMBER: 37

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Syllabus

PART I. QUALITATIVE PROCEDURES llAGE
General Instructions 1
EXEIWISE I. Det~ction of the Elements m Animal and Plant
Material 2
EXERCISE II. The, Carbohydrates . 7
EXERCISE III. Saliva and Salivary Amylabc 25
EXERCISE IV. Lipides and their Derivatives 31
EXERCISE V. Amino-acids and Proteins. 37
EXERCISE VI. Gastric, PancreatIC and Intestinal DigestIOn 47
EXERCISE VII. Qualitative Examination of l\hlk 65
EXERCISE VIII. Scheme for the Examination of Unknown
Protems and Protein Derivatives 69
EXERCISE IX. Qualitative Exammation of Foodstuffs 72
EXERCISE X. Blood 73
EXERCISE XI. Respiration . 85
EXERCISE XII. The CompOSition of the Excreta 89
EXERCISE XIII. The Chemical Composition of the Tissues 9S
EXERCISE XIV. Examination of Unknown Compounds of
Biochemical Importance . 106
PART II. QUANTITATIVE AND CLINICAL

PROCEDURES

EXERCISE XV. Cleaning, Care and Standardisation of
Apparatus for Quantitative Work IDS
EXERCISE XVI. The Estimation of Nitrogen by Kjeldahl’s
Method . ‘114
EXERCISE XVII. The Colorimeter and the Estimation of
Creatinine and Creatine in Urine 118

EXERCISE XVIII. Estimation of Urine Acidity by Titra-
tion and by pH Determination; EstimatIon of

Ammonia in Urine. 125
EXERCISE XIX. Estimation of Glucose in Pure Solution
·and in Urine . 132

EXERCISE XX. Estimation of Ammonia and Urea in Urine 135
EXERCIS:C XXI. Estimation of Chlorides in Urine . 139
EXERCISE XXII. Estimation of Phosphates in Urine 141
EXERCISE XXIII. Examination of Pathological Urines 143
EXERCISE XXIV. Gastric Analysis and Analysis of Gastric
Contents 148
EXERCISE XXV. Preparation of Protein-free Blood
Filtrates and their Analysis . 154
Estimation of Blood Glucose . 158-
Determination of Sugar Tolerance 161
Estimation of Blood Creatinine 163

EXERCISE XXVI, Estimations of Whole Blood not in-
volving Protein ;E’redpitation and of Non-protein

Nitrogen 166
Estimation of Urea. 166
Estimation of Non-Protein Nitrogen. 168
Estimation of Cholesterol 168
EXERCISE XXVII. Estimation of Certain Blood Serum
Constituents 171
Calcium 171
Phosphatase . 173
EXERCISE XXVIII. Estimations of Certain Blood Plasma
Constituents 178
Estimation of Chlorides. . 178
Estimation of Inorganic Phosphate 181
Estimation of Plasma Proteins 182
EXERCISE XXIX. Determination of Blood Bases (so-called
Alkali Reserve) 186
EXERCISE XXX. Determination of Basal Metabolic Rate 195

EXERCISE XXXI. Chemistry of Vitamins and Determina-
tion of a Growth Curve . 201

EXERCISE XXXII. Examination of a Twenty-four Hours’
Specimen of Urine . 205

EXERCISE XXXIII. Applications of Biochemical Pro-
cedures to Measurement of Certain Tissue Functions 208

Tests of Liver Function . 208
Tests of Kidney Function 213
Tests of Pancreatic Function 218
ApPENDICES .
i. Strengths of Solutions and Special Reagents 222
ii. Useful Data . . 229
iii. First Aid Procedures . 232
INDEX. 236

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