165. Pharmacognosy & Pharmacological Studies

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165. Pharmacognosy & Pharmacological Studies

165. Pharmacognosy & Pharmacological Studies

 

 

CATEGORY: Medical & Medicine – 500 Courses

COURSE NUMBER: 165

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BOOKS/ MANUALS: Pages

Syllabus

1. Introduction 1-11
1.1. Pharmacognosy
1.2. Phytochemistry
1.2.1. Phytochemical Revolution
1.3. Pharmacology
1.3.1. Antioxidant Activity
1.3.2. Anti – Depressant Activity
1.3.3. Anti – Diabetic Activity
2. Review of Literature 11-18
2.1. Family Rubiaceae
2.2. Objectives of the Present Study
3. Materials and Methods 19-50
3.1. Collection of Plant material
3.2. Taxonomy of the Species
3.3. Morphological Features
3.4. Ecology
3.5. Medicinal Uses
3.6. Chemical constituents isolated from the different species of Tricalysia
3.7. Pharmacognostical Studies
3.7.1. Anatomical studies
3.7.2. Histochemical Colour Reactions
3.7.3. Fluorescence Analysis
3.8. Phytochemistry
3.8.1. Physio – Chemical Constants
3.8.2. Preparation of the Extracts
3.8.3. Extractive Values
3.8.4. PH Determination of Powdered Drug
3.8.5. Preliminary Phytochemical Screening
3.8.6. Gas Chromatography-Mass Spectrometry (GC-MS) Analysis
3.9. Pharmacology
3.9.1. In vitro Antioxidant activity
3.9.1.1. Inhibitiory effects on DPPH Radical Assay
3.9.1.2. Hydrogen peroxide Assay
3.9.1.3. Superoxide dismutase (L-methionine and NBT) assay

3.9.1.4. Iron Chelating Activity (FRAP)
3.9.2. In vivo Pharmacological Studies
3.9.2.1. Acute Toxicity Studies
3.9.2.2. Anti-Depressant Activity
3.9.2.2.1. Forced Swimming Test (FST)
3.9.2.2.2. Tail Suspension Test (TST)
3.9.2.2.3. Hole Board Test (HBT)
3.9.2.3. Anti Diabetic Activity
3.9.2.3.1. Screening of Hypoglycemic Activity in Normal Rats
3.9.2.3.2. Anti-Diabetic Activity in Experimentally Induced Diabetic Rats
3.9.2.3.2.1. Single-Dose Short Term Study
3.9.2.3.2.2. Multi- Dose Long Term Study
3.9.2.3.3. Effect of Formulation Test Extract on Body Weight in Normal and
Alloxan Induced Diabetic Rats
3.9.2.3.4. Biochemical Parameters Determinations
3.9.2.3.5. Histopathological Studies
4. Results 51-76
4.1. Pharmacognosy
4.1.1. Anatomy
4.1.1.1. Leaf peeling
4.1.1.2. Venation Pattern
4.1.1.3. Quantitative Values of Foliar Epidermis
4.1.1.4. Stem Peeling
4.1.1.5. Maceration
4.1.1.6. Transverse Section of Leaf
4.1.1.7. Transverse Section of Stem
4.1.1.8. Transverse Section of Root
4.1.2. Histochemical Colour Reactions
4.1.3. Fluorescence Analysis
4.2. Phytochemistry
4.2.1. Physico-Chemical Parameters of Various Parts
4.2.2. Extractive Values
4.2.2.1. Batch Process
4.2.2.2. Successive Process
4.2.3. PH Determination of Powdered Drug
4.2.4. Preliminary Phytochemical screening
4.2.5. GC-MS Analysis
4.2.5.1. GC-MS Analysis of Methanolic Extract of Leaf

4.2.5.2. GC-MS Analysis of Methanolic Extract of Stem
4.2.5.3. GC-MS Analysis of Methanolic Extract of Root
4.2.5.4. GC-MS Analysis of Methanolic Extract of Fruit
4.2.5.5. Comparative Analysis of Compounds Identified by GC-MS Analysis
4.3. Pharmacology
4.3.1. Invitro Antioxidant Activity
4.3.1.1. DPPH Scavenging Activity
4.3.1.2. Iron Chelating Activity (FRAP)
4.3.1.3. Hydrogen peroxide Assay
4.3.1.4. Superoxide dismutase (L-methionine and NBT) assay
4.3.2. In vivo Pharmacological Studies
4.3.2.1. Acute Toxicity Studies
4.3.2.2. Anti-Depressant Activity
4.3.2.2.1. Forced Swimming Test (FST)
4.3.2.2.2. Tail Suspension Test (TST)
4.3.2.2.3. Hole Board Test (HBT)
4.3.2.3. Anti Diabetic Activity
4.3.3.1. Screening of Hypoglycemic Activity in Normal Rats
4.3.3.2. Anti-Diabetic Activity in Experimentally Induced Diabetic Rats
4.3.3.2.1. Single-Dose Short Term Study
4.3.3.2.2. Multi- Dose Long Term Study
4.3.3.3. Effect of Formulation Test Extract on Body Weight in Normal and
Alloxan Induced Diabetic Rats
4.3.3.4. Biochemical Parameters Determinations
4.3.3.5. Histopathological Studies
5. Discussion 77-93
5.1. Pharmacognosy
5.2. Phytochemistry
5.2.1. Phytochemical Screening
5.2.2. GC-MS Analysis
5.3. Pharmacognosy
5.3.1. Antioxidant Activity
5.3.2. Anti – Depressant Activity
5.3.3. Anti – Diabetic Activity
6. Summary and Conclusions 94-98
7. Bibliography 99-112
8. Publications

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