114. Lipid Analysis In Oils & Fats

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114. Lipid Analysis In Oils & Fats

 

 

CATEGORY: Diet Nutrition Supplementation – 500 Courses

COURSE NUMBER: 114

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Syllabus

Contributors xi
Preface xvii
Volumes in Series xix
1. Qualitative Analysis and Quantitative Assessment of Changes in
Neutral Glycerol Lipid Molecular Species Within Cells 1
Jessica Krank, Robert C. Murphy, Robert M. Barkley, Eva Duchoslav, and
Andrew McAnoy
1. Introduction 2
2. Reagents 3
3. Methods 4
4. Results 7
5. Conclusions 19
Acknowledgments 19
References 19
2. Glycerophospholipid Identification and Quantitation by Electrospray
Ionization Mass Spectrometry 21
Pavlina T. Ivanova, Stephen B. Milne, Mark O. Byrne, Yun Xiang, and
H. Alex Brown
1. Introduction 22
2. Nomenclature 25
3. Mass Spectrometry 26
4. General Strategy for Phospholipid Isolation and Mass Spectral Analysis 27
5. Extraction and Mass Spectral Analysis of Global Glycerophospholipids 28
6. Polyphosphoinositide Extraction and Mass Spectral Analysis 35
7. Computational Analysis of Mass Spectral Data 41
Acknowledgments 54
References 54

3. Detection and Quantitation of Eicosanoids via High Performance
Liquid Chromatography-Electrospray Ionization-Mass Spectrometry 59
Raymond Deems, Matthew W. Buczynski, Rebecca Bowers-Gentry,
Richard Harkewicz, and Edward A. Dennis
1. Introduction 60
2. Methods 62
3. Results and Discussion 74
Acknowledgments 80
References 81
4. Structure-Specific, Quantitative Methods for Analysis of Sphingolipids
by Liquid Chromatography–Tandem Mass Spectrometry:
‘‘Inside-Out’’ Sphingolipidomics 83
M. Cameron Sullards, Jeremy C. Allegood, Samuel Kelly, Elaine Wang,
Christopher A. Haynes, Hyejung Park, Yanfeng Chen, and Alfred H. Merrill, Jr.
1. Introduction: An Overview of Sphingolipid Structures and Nomenclature 86
2. Analysis of Sphingolipids by Mass Spectrometry 89
3. Analysis of Sphingolipids by ‘‘OMIC’’ Approaches 92
4. Materials and Methods 95
5. Materials 98
6. Extraction 98
7. Identification of the Molecular Species by Tandem Mass Spectrometry 100
8. Quantitation by LC-ESI-MS/MS Using Multireaction Monitoring 101
9. Analysis of (Dihydro)Ceramides, (Dihydro)Sphingomyelins, and
(Dihydro)Monohexosyl-Ceramides in Positive Ion Mode 104
10. Other Methods 109
Acknowledgments 111
References 112

5. Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate-
Oligosaccharides by Liquid Chromatography-Electrospray

Ionization-Mass Spectrometry 117
Teresa A. Garrett, Ziqiang Guan, and Christian R. H. Raetz
1. Introduction 118
2. Materials 121
3. Liquid Chromatography-Mass Spectrometry 122
4. Preparation of Lipid Extracts 122
5. LC-MS Detection and Quantification of Coenzyme Q 123
6. LC-MS Detection and Quantification of Dolichol 130

7. LC-MS and LC-MS/MS Characterization of Dolichol
Diphosphate-Linked Oligosaccharides 136
Acknowledgment 140
References 140
6. Extraction and Analysis of Sterols in Biological Matrices by High
Performance Liquid Chromatography Electrospray Ionization
Mass Spectrometry 145
Jeffrey G. McDonald, Bonne M. Thompson, Erin C. McCrum, and
David W. Russell
1. Introduction 146
2. Supplies and Reagents 148
3. Extraction of Lipids from Cultured Cells and Tissues 149
4. Saponification of Lipid Extracts 151
5. Solid-Phase Extraction 152
6. Analysis by HPLC-ESI-MS 153
7. Quantitation 156
8. Data 157
9. Discussion, Nuances, Caveats, and Pitfalls 162
Acknowledgments 168
References 169
7. The Lipid Maps Initiative in Lipidomics 171
Kara Schmelzer, Eoin Fahy, Shankar Subramaniam, and Edward A. Dennis
1. Introduction 172
2. Building Infrastructure in Lipidomics 173
3. Classification, Nomenclature, and Structural Representation of Lipids 174
4. Mass Spectrometry as a Platform for Lipid Molecular Species 177
5. Future Plans 180
Acknowledgments 182
References 182
8. Basic Analytical Systems for Lipidomics by Mass Spectrometry
in Japan 185
Ryo Taguchi, Mashahiro Nishijima, and Takao Shimizu
1. Introduction 186
2. Lipid Bank and Related Databases 187
3. Strategies for Lipid Identification and Quantitative Analysis by
Mass Spectrometry 188

4. Several Practical Lipidomics Methods by Mass Spectrometry 190
5. Strategies for Identification of Individual Molecular Species in
Glycerolipids and Glycerophospholipids by Lipid Search 192
6. Quantitative Analysis or Profiling of Lipid Molecular Species 197
7. Application of Several Different Methods in Lipidomics 199
8. Future Program for Lipidomics 208
Acknowledgments 209
References 209
9. The European Lipidomics Initiative: Enabling Technologies 213
Gerrit van Meer, Bas R. Leeflang, Gerhard Liebisch, Gerd Schmitz, and
Felix M. Gon ̃i
1. Introduction 214
2. Methods of Lipidomics: Quantitative Analysis in Time 215
3. Imaging Lipids 218
4. Methods to Study the Physical Properties of Lipids 219
5. Data Handling and Standardization 224
6. Perspectives 226
Acknowledgments 227
References 227
10. Lipidomic Analysis of Signaling Pathways 233
Michael J. O. Wakelam, Trevor R. Pettitt, and Anthony D. Postle
1. General Considerations 235
2. Lipid Extraction 235
3. HPLC-MS Analysis 237
4. General Phospholipid HPLC 237
5. Ceramide, Diradylglycerol, and Monoradylglycerol Separation 239
6. Phosphoinositide Separation 240
7. Analysis of Phospholipid Synthesis by ESI-MS/MS Using
Stable Isotopes 241
8. Labeling Protocols 242
9. Lipid Extraction for ESI-MS/MS 243
10. LC-MS Analysis (methyl-d9-)-Choline 243
11. MRM Analysis of (methyl-d9)-Choline Enrichment in Cell and
Tissue Phosphorylcholine 244
12. ESI-MS/MS Analysis of Native and Newly Synthesized Phospholipids 244
13. Data Analysis 245
References 246

11. Bioinformatics for Lipidomics 247
Eoin Fahy, Dawn Cotter, Robert Byrnes, Manish Sud, Andrea Maer, Joshua Li,
David Nadeau, Yihua Zhau, and Shankar Subramaniam
1. Introduction 248
2. Lipid Structure Databases 249
3. Lipid-Associated Protein/Gene Databases 254
4. Tools for Lipidomics 256
5. Lipid Pathways 268
6. Challenges for Future Lipid Informatics 270
Acknowledgments 272
References 272
12. Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins,
and Protectins 275
Charles N. Serhan, Yan Lu, Song Hong, and Rong Yang
1. Introduction: Metabolomics and Mediator Lipidomics–Informatics 276
2. Engineered Animals and Human Tissues 278
3. New Chemical Mediator Pathways in Resolution of Inflammation 279
4. Logic Diagram to Identify PUFA-Derived Lipid Mediators: Eicosanoids,
Resolvins, and Protectins 284
5. Cognoscitive-Contrast-Angle Algorithm 286
6. Theoretical Database for Novel LM and Search Algorithm 292
7. Discussion 308
8. Conclusions and Next Steps 311
Acknowledgments 313
References 313
13. A Guide to Biochemical Systems Modeling of Sphingolipids for
the Biochemist 319
Kellie J. Sims, Fernando Alvarez-Vasquez, Eberhard O. Voit, and
Yusuf A. Hannun
1. Introduction 320
2. System Map: Specifying the Model 322
3. Symbolic Equations: From Words and Pictures to Equations 327
4. Numerical Equations: From the Symbolic to a Computational Model 331
5. Model Analysis: Steady State, Stability, and Sensitivity 341
6. Simulation: What Happens If…? 345
7. Conclusion 346

8. Epilogue 346
Acknowledgments 348
References 348
14. Quantitation and Standardization of Lipid Internal Standards for
Mass Spectroscopy 351
Jeff D. Moore, William V. Caufield, and Walter A. Shaw
1. Introduction 352
2. Lipid Handling Guidelines 352
3. Chemical Characterization of Lipid Stocks 353
4. Preparation of Working Lipid Standards 355
5. Packaging of Lipid Standards 359
6. Quality Control and Stability Testing 361
7. Discussion 364
References 366
Author Index 369
Subject Index 381

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